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KMID : 1101420230550030184
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Korean Journal of Clinical Laboratory Science
2023 Volume.55 No. 3 p.184 ~ p.194
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Role of p-anisaldehyde in the Differentiation of C2C12 Myoblasts
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Kim Dal-Ah
Kong Kyoung-Hye
Cho Hyun-Jeong
Lee Mi-Ran
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Abstract
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In this study, we investigated whether p-anisaldehyde (PAA), the main component of essential oils derived from anise seeds, influences the differentiation of mouse C2C12 myoblasts. Cells were induced to differentiate over 5 days using a differentiation medium with or without PAA (50 or 200 mg/mL). Myotube length and diameter were measured, and the expressions of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain) and atrophy-related genes (atrogin-1 and muscle ring finger-1 [MuRF-1]) were assessed by quantitative real-time polymerase chain reaction. Additionally, protein kinase B (Akt) phosphorylation was monitored by western blotting. PAA significantly induced the formation of smaller and thinner myotubes and reduced myogenic marker expression. Furthermore, PAA increased the expressions of atrogin-1 and MuRF-1 and simultaneously reduced Akt phosphorylation. Our findings indicate that PAA inhibits the myogenic differentiation of C2C12 cells by reducing the phosphorylation and activation of Akt.
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KEYWORD
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Atrophy, Myogenesis, p-anisaldehyde, Skeletal muscle
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